4 ways to reduce the interference of lipid samples with biochemical analysis

release time:2021-11-22 15:36:50

In the previous article, we learned what lipidemia is and how it affects biochemical test results. So what should we do? How can we reduce the interference of lipid samples on biochemical analysis results? Four methods are described below.

1. Treatment of lipid samples with ether

To exclude the interference of lipid blood, consider the option of treating lipid blood samples with ether. The effect of lipid clouding on ALT, AST, and TP is excluded. However, the interference of lipid clouding on ALB, GGT, LDH, BUN, GLU still exists. Moreover, the effect of lipid samples on the results of TBIL, DBIL, T-G, and GLU was higher.

2. Diluted sample reassay

When the results of lipid blood samples exceed the linear range, dilution of samples can be considered for re-testing. However, this is prone to error. In addition, the effect of lipid blood on biochemical assay results can be reduced by treating the samples with PEG.

3. Chemical treatment methods

It has been shown that treatment of lipid samples with 6.67 g/dL PEG can reduce the effect of lipid blood on biochemical assay results. Treatment of lipid blood samples with ether can only exclude the effect of some indicators.

4. Physical treatment methods

Clinical lipid samples were separated from serum by low-speed centrifugation (367-573 Xg, 5 min) for determination. The serum was sealed with a cap after routine centrifugation. After high speed centrifugation (7155 Xg, 8 min), the celiac layer is separated and the serum is aspirated for determination. This method reduces the interference of lipid blood on ALT, AST, GGT, CRE and TP biochemical indexes. The lower clear layer after high speed centrifugation inevitably leads to a decrease in T-CHOL and T-G. In particular, the concentration of T-G will be significantly decreased, therefore, this method is not suitable for the detection of these two indicators.

For severely lipidated samples, the veterinarian needs to explain to the pet owner. It is best to re-collect samples for testing when conditions are suitable. Testing should be stopped for severely affected lipidemic samples.

For biochemical test results that are mildly affected by lipemia, explain to the pet owner that the sample has mild lipemia. The measured biochemical index is for reference only. It cannot be used as a complete diagnostic basis. Diagnostic analysis should also be performed in conjunction with clinical signs and other ancillary diagnostic equipment. The veterinarian should also indicate the lipemia and its severity on the test report form.

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