What are the factors that affect the accuracy of a pet biochemical analyzer?

release time:2021-04-21 10:50:27

Pet biochemical test is an indispensable tool for veterinarians to diagnose diseases, but there are many factors that affect the accuracy of the results. This article summarizes some influencing factors.

1. Animal factors: Some of the pet's own factors will have a certain impact on the test results, such as the pet's age, gender, and degree of obesity.

Mood: Transportation and blood sampling of pets will cause stress reactions in animals, especially for cats, the concentration of serum non-esterified fatty acids, lactic acid, blood sugar, etc. will increase. Before blood sampling, pets should be comforted to reduce stress.

Movement: Strenuous exercise will cause the pet's ALT, AST, LDH, CK, and other indexes to increase over a period of time. In order to reduce the influence of exercise factors on the result, you can try to collect blood for your pet in the morning and avoid strenuous exercise before blood collection. After strenuous exercise, let him rest for at least 30 minutes before taking blood.

Diet: Within a few hours after a meal, the pet's blood sugar, blood urea ammonia, uric acid, triglyceride, and other indexes will increase; pets fasting for too long will cause blood sugar, protein to decrease, and bilirubin to increase.

Generally, the blood test is performed 4 hours after the animal has eaten, and the blood lipid test should be performed after the animal fasts for 12 hours.

Infusion: The long-term infusion will dilute the animal's blood sample, causing certain indexes to below. During intravenous injection, high-concentration drugs enter the blood sample, which will cause great changes in many test results. Therefore, samples should be taken 2 hours after the end of the infusion.

2.Hemolysis: The red blood cells in the blood are destroyed and released into the blood to make the serum or plasma appear red.

The production of hemolysis samples is mostly caused by the excessively long time of sample placement, improper blood collection, difficulty in blood collection, and mechanical shock. There are fewer cases of hemolysis or pathological hemolysis in the blood vessels of animals.

3. Lipemia

The lipid content enters the blood, making the serum or plasma turbid and milky white.

Compared with hemolysis, the probability of lipemia in the sample is higher, and this condition is mostly found in the blood of obese pets. The main manifestations of lipemia are the interference with absorbance and the non-uniformity of the analyte distribution.

4. Drug effects

The drug or its metabolites can affect any step of the assay.

①Drugs that affect blood sugar
②The effect of vitamin C on some test results

5. After blood collection, the sample was left for too long. The timely and correct handling of specimens is very important to the accuracy of the results.

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What should I do if I encounter an abnormal sample during pet biochemical testing?

2021-04-22

Compared to human medical testing, abnormal samples account for a larger number of blood samples that can be collected by veterinarians. When collecting blood, what should be done with abnormal samples such as lipemia, hemolysis, jaundice and coagulation?