In recent years, with the cross-fertilization of biological high technology and laboratory medicine, many high-end and highly automated new immunoassay equipment have been introduced into the clinical testing field one after another. This has greatly advanced the development of immunological testing, such as fully automated fluorescence immunoassay analyzer, enzyme-linked immunoassay analyzer, chemiluminescence immunoassay analyzer, etc.
These new immunological analysis equipment has greatly increased the sensitivity and specificity of the analysis index, and also significantly improved the accuracy and efficiency of the test. In particular, the fully automated chemiluminescence immunoassay analyzer has become the most widely used and actively developed test equipment in clinical testing.
Chemiluminescence immunoassay is a combination of chemiluminescence assay technology with high sensitivity and immune reaction with high specificity for the detection and analysis of various antigens, semi-antigens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs.
Chemiluminescence immunoassay is one of the latest immunoassay techniques developed after radioimmunoassay, enzyme immunoassay, fluorescence immunoassay and time-resolved fluorescence immunoassay.
6 advantages of chemiluminescence immunoassay analyzer
1, High sensitivity of immunoassay analyzer
Sensitivity is the key to the superior performance of immunoassay analyzer. The sensitivity of some analyzers can reach 10 -16 mol / L (RIA for 10-12 mol / L). Another example is chemiluminescent substrates (such as AMPPD), the concentration of alkaline phosphatase can be detected than the chromogenic substrate to be sensitive 5 х 10 ^ 5 times.
2. Wide linear kinetic range
The luminescence intensity of chemiluminescence immunoassay is linear between 4 and 6 orders of magnitude with respect to the concentration of the measured substance. This is a significant advantage compared to the absorbance (OD) range of 2.0 for colorimetric enzyme immunoassays. Although RIA also has a wide range of linear kinetics, radioactivity limits its application.
3, Long duration of light signal
The glow type CLIA produces a light signal that lasts for hours or even a day. This certainly simplifies the experimental operation and measurement.
4, The analysis method is simple and fast
Most of the chemiluminescence immunoassay analyzers are one-step mode that only requires the addition of one reagent (or a combination of reagents).
5, Stable results, small error
The sample directly emits light on its own, without any light source irradiation, eliminating the impact of various possible factors (light source stability, light scattering, light wave selector, etc.) to the analysis. This makes the analysis results sensitive, stable and reliable.
6. Good safety and long service life
Chemiluminescent immunoassay analyzer eliminates the use of radioactive substances. So far, it has not been found to be harmful. Moreover, the reagents used for immunoassay are stable and can be stored for up to one year.
The Seamaty
SMT-680V fully automated veterinary immunoassay analyzer is easy to operate and requires only a few steps to complete compared to the complex operation of large immunoassay analyzers. The analyzer uses the complete steps of laboratory chemiluminescence and has a pico-gram accuracy with a CV <8%.
